human skin fibroblasts hs 27 cell line  (ATCC)

Journal: Journal of Dental Sciences

Article Title: Ovatodiolide overcomes cisplatin resistance in head and neck cancer by disrupting a novel oncogenic signature and cancer-associated fibroblast activation

doi: 10.1016/j.jds.2025.08.041

Figure Lengend Snippet: Cancer-associated fibroblasts-derived signals enhance chemoresistance, stemness, and metastatic potential in tumor cells. Dose-response curves illustrate the effect of cisplatin on the viability of CAL-27 and Ca9-22 HNSCC cells (A) and tumor spheroids (B). IC50 values are indicated. (C) Transformation of cancer-associated fibroblast (CAF)-like phenotype in WS1 fibroblasts by tumor-conditional medium (TCM). Immunofluorescence staining for vimentin (Vim) and α-smooth muscle actin (α-SMA) is shown. Right panel: Quantitative RT-PCR analysis of α-SMA, Vim, and TGFB1 mRNA expression in WS1 cells treated with TCM versus control. (D) Representative images (left) and quantification (right) of spheroid formation by CAL-27 and Ca9-22 cells cultured in control medium or CAF-conditioned medium (CAF-CM). Only spheroids with a diameter of >200 μm were tabulated. (E) qPCR analysis of tumor spheroids generated under control and CAF-CM conditions. Relative mRNA expression of the SIS signature (SERPINE1, INHBA, SPP1), metastasis (MMP1, MMP3), and stemness (CD44, YAP1) in HNSCC spheroids generated from control medium or CAF-CM, as determined by quantitative RT-PCR. TCM, tumor-conditional medium. CAF-CM, cancer-associated fibroblast conditioned medium. ∗∗∗ P < 0.001.

Article Snippet: Head and neck cancer cell lines (CAL27 and Ca9-22) and human skin fibroblast cells (WS1) were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA) and cultured according to the supplier's recommended conditions.

Techniques: Derivative Assay, Transformation Assay, Immunofluorescence, Staining, Quantitative RT-PCR, Expressing, Control, Cell Culture, Generated

Journal: Journal of Dental Sciences

Article Title: Ovatodiolide overcomes cisplatin resistance in head and neck cancer by disrupting a novel oncogenic signature and cancer-associated fibroblast activation

doi: 10.1016/j.jds.2025.08.041

Figure Lengend Snippet: Ovatodiolide treatment reduces tumorigenesis, cancer stemness, and cancer-associated fibroblast transformation. (A) Dose-response curves showing ovatodiolide cytotoxicity in CAL27 and Ca9-22 cells (48 h). IC50 values are indicated in the box. (B) qPCR and Western blot analysis of SIS oncogenic signature genes (SERPINE1, INHBA, SPP1, MMP3, YAP1) in ovatodiolide-treated CAL27 and Ca9-22 cells. GAPDH served as a loading control. (C) Sphere formation assay showing reduced stemness after ovatodiolide treatment. Left: representative images of spheroids. Right: quantification of sphere numbers. Scale bar = 200 μm. (D) CAF transformation analysis. Left: Western blot of CAF markers (α-SMA, vimentin, TGF-β1) in WS1 fibroblasts treated with tumor-conditional medium (TCM) or ovatodiolide-treated TCM (Ovato + TCM). Right: Green immunofluorescence of α-SMA and vimentin. Scale bar = 50 μm ∗ P < 0.05, ∗∗ P < 0.01.

Article Snippet: Head and neck cancer cell lines (CAL27 and Ca9-22) and human skin fibroblast cells (WS1) were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA) and cultured according to the supplier's recommended conditions.

Techniques: Transformation Assay, Western Blot, Control, Tube Formation Assay, Immunofluorescence

Journal: Sensors (Basel, Switzerland)

Article Title: A Heptamethine Cyanine-Based Near-Infrared Optical Sensor for Copper(II) Detection in Aqueous Solutions and Living Cells

doi: 10.3390/s26010130

Figure Lengend Snippet: Confocal microscopy images of WS1 fibroblast cells. Bright-field image ( A ) and confocal fluorescence image ( B ) of WS1 cells incubated with IRPhen (10 µM) for 30 min. Bright-field image ( C ) and confocal fluorescence image ( D ) of cells pretreated with Cu 2+ (10 µM) for 8 h, followed by incubation with IRPhen (10 µM) for 30 min; ( E ) Quantitative bar graph showing the corresponding fluorescence intensities of panels ( B , D ). Excitation was at 633 nm, and emission was collected from 650–850 nm. Scale bar, 10 µm.

Article Snippet: Human skin primary fibroblast cells (WS1) purchased from American Type Culture Collection (ATCC, Manassas, VA, USA) were used in this study.

Techniques: Confocal Microscopy, Fluorescence, Incubation